Research progress of phosphopeptide separation and enrichment methods
Aims:By reviewing the research progress of enrichment methods for O-phosphorylated peptide segments and N-phosphorylated peptide segments,a solution to the pretreatment of phosphorylated protein analysis was provided.Methods:Based on literature and database searching,the basic principles and applications of the enrichment methods for O-phosphorylated and N-phosphorylated peptides were summarized;and the research progress of the enrichment methods for O-phosphorylated and N-phosphorylated peptides was introduced systematically.Results:The separation and enrichment method of phosphorylated peptide segments had been widely applied in the study of phosphorylated proteomics.Conclusions:At present,various enrichment methods have been developed for different phosphorylated peptide segments;and each method has different specificity and selectivity.Therefore,it is particularly important to choose enrichment methods based on research objectives.
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