Effects of Lonicera flos and Cnicus japonicus Extracts on Production Performance,Follicular Development,Hepatic Antioxidant Capacity and Splenic Immune Function of Laying Hens at the Late Laying Period
In order to study the effects of Lonicera flos and Cnicus japonicus extracts(LCE)on production performance,follic-ular development,hepatic antioxidant capacity and splenic immune function of laying hens at the late stage of laying,a total of 73-week-age 1 728 Lohmann Pink laying hens were selected and randomly divided into four groups with 18 replicates per group and 24 hens in each replicate.The layers in the control group were fed a basal diet,and layers in the experiment groups were fed basal diets supplemented with 300,500 and 1 000 mg/kg LCE,respectively.The results showed that:at 78 weeks of age,com-pared with the control group,300 and 1 000 mg/kg LCE significantly increased the serum uric acid content(P<0.05);300 and 500 mg/kg LCE significantly decreased serum LH content(P<0.05);The 500 and 1 000 mg/kg LCE significantly decreased se-rum estradiol content(P<0.05);The 300 mg/kg LCE significantly reduced the number of follicles with the diameter at 5 to 10 mm(P<0.05);The 500 mg/kg LCE significantly decreased the catalase activity and malondialdehyde content in liver and the relative mRNA expression of splenic iNOS(P<0.05);At 83 weeks of age,compared with the control group,1 000 mg/kg LCE significantly increased serum uric acid content(P<0.05);The 500 and 1 000 mg/kg LCE significantly increased serum luteinizing hormone content(P<0.05);The 300 mg/kg LCE significantly increased hepatic catalase activity(P<0.05).In conclusion,500 mg/kg LCE had no adverse effects on production performance of laying hens in the late laying period,and could enhance the antioxidant ca-pacity of the liver and suppresse the inflammatory response of the spleen.
Lonicera flos and Cnicus japonicus extractslaying henfollicular developmentantioxidant capacityimmune function