Accuracy of Early Identification of Chicken Embryo Sex by Estradiol and Testosterone Concentrations in Allantoic Fluid
To explore whether sex hormones in chicken embryo oocyst fluid can be used as markers for chicken embryo sex identification,100 chicken embryos were divided into 5 groups and incubated for 8,10,12,14 and 16 days respectively.The oo-cyst fluid and muscle tissue were extracted,and the changes of sex hormone content in chicken embryo oocyst fluid were detected by radioimmunoassay(RIA).The chicken embryo sex was accurately identified by PCR technology,and the differences of estradi-ol and testosterone concentrations in female and male chicken embryo oocyst fluid were analyzed.The results showed that the con-centrations of estradiol(E2)and testosterone(T)increased continuously with the increase of days.The concentrations of E2 and T in male embryos increased first and then slightly decreased,while the concentrations of the two sex hormones in female embryos increased continuously.The difference of estradiol concentration between male and female chicken embryos at 16 days was ex-tremely significant(P<0.01),and the difference of testosterone concentration was significant(P<0.05).By comparing with the re-sults of chicken embryo sex chromosome CHD gene detection by PCR method,the consistency rate of RIA method with estradiol as a marker for predicting the sex of chicken embryos at 16 days was 96.5%,and the consistency rate of RIA method with testos-terone as a marker was 89.6%,indicating that estradiol was more suitable as a hormone marker for early identification of chicken embryo sex.
estradioltestosteronesex hormoneprediction of chicken embryo sexaccuracy
万方数据
维普
