Preparation and Identification of Specific Monoclonal Antibody Against Lipopolysaccharide of Riemerella anatipestifer
In order to prepare specific monoclonal antibody(mAb)targeting the lipopolysaccharide(LPS)of Riemerella anatipestifer(RA),BALB/c mice were vaccinated with inactivated bacteria of RA SDJ-80 strain,and the mAb specific to LPS was prepared by fusing myeloma cells SP2/0 with immunized positive mouse spleen cells.The LPS was used as the coating antigen,and positive clones were screened using indirect ELISA method,then subjected to sub-cloning.The prepared positive hybridoma cell line was continuous passaged for 10 generations,and the titer and specificity of the mAb were detected.The results showed that a mAb named as 1E6 was obtained successfully,the titer of which was higher than 1:204 800 and could react specifically with RA serotype 1,2,6,7,and 10 strains,which could be used for future study on RA LPS structure and novel diagnostic methods.
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