新型便携式体外膜肺氧合动物实验在体安全性和有效性研究
Study on the safety and efficacy of novel portable extracorporeal membrane oxygenation in animal experiments in vivo
翟蒙恩 1罗建超 1卢林鹤 1任雨超 1金屏 1刘振华 2杨剑 1金振晓 1刘金成 1刘洋1
作者信息
- 1. 中国人民解放军空军军医大学第一附属医院心血管外科,陕西西安 710032
- 2. 中国人民解放军空军军医大学卫勤训练基地,陕西西安 710032
- 折叠
摘要
目的 本研究旨在验证一种自主研发的新型便携式体外膜肺氧合(ECMO)系统(西京高级生命支持系统JC-Ⅲ型)在大动物体内的安全性和有效性.方法 共计10只健康小尾寒羊通过颈动脉-颈静脉插管方式进行静脉-动脉体外膜肺氧合(VA-ECMO)支持,以评价JC-Ⅲ型ECMO系统的性能.通过持续输注肝素实现全身抗凝,转机过程中每2 h记录1次活化凝血时间(ACT),维持ACT在200～250 s之间;离心泵转速设定在3 000～3 500 r/min.分别于ECMO启动前和启动后24 h监测血红蛋白、血细胞计数、凝血、肝肾功能、心肌损伤等指标变化情况,实验结束后解剖泵头和氧合器,探查血栓形成情况.结果 VA-ECMO手术成功率为100％,ECMO转机24 h,流量波动于1.9～2.5 L/min,未发生溶血、泵头血栓、膜肺血栓.ECMO转机前后血红蛋白含量、白细胞计数、血小板计数、谷丙转氨酶、谷草转氨酶、尿素、肌酐、高敏肌钙蛋白Ⅰ、N末端B型脑钠肽前体等指标差异均无统计学意义(均P＞0.05).结论 本在体动物实验证实了西京高级生命支持系统JC-Ⅲ型的安全性和有效性.
Abstract
Objective To verify the safety and efficacy of a new portable extracorporeal membrane oxygenation(ECMO)system(Xijing Advanced Life Support System JC-Ⅲ)in large animals.Methods A total of 10 healthy small fat-tail sheep underwent veno-arterial extracorporeal membrane oxygenation(VA-ECMO)support by carotid arterial-jugular catheterization to evaluate the performance of the JC-Ⅲ ECMO system.Systemic anticoagulation was achieved by continuous infusion of heparin.Active coagulation time(ACT)was recorded every 2 hours during the experiment,and the ACT was maintained between 200-250 s.Centrifugal pump speed is set at 3 000-3 500 r/min.The changes of hemoglobin,blood cell counts,hematocrit,liver and kidney function were monitored before and 24 h after ECMO initiation,respectively.After the experiment,the pump and oxygenator were dissected to probe the thrombosis.Results The success rate of VA-ECMO operation was 100％,and there was no hemolysis,pump thrombosis and oxygenator thrombosis after 24 h of ECMO.Before and after the operation,there were no significant changes in indicators such as hemoglobin content,white blood cell counts,platelet counts,alanine aminotransferase concentration,aspartate aminotransferase concentration,urea,creatinine,high-sensitivity troponin Ⅰ,and N-terminal pro-brain natriuretic peptide(all P＞0.05).Conclusions This in vivo study confirms that Xijing Advanced Life support System JC-Ⅲ is safe and effective.
关键词
便携式体外膜肺氧合/西京高级生命支持系统JC-Ⅲ型/大动物实验/安全性/有效性Key words
Portable extracorporeal membrane oxygenation/Xijing Advanced Life Support System JC-Ⅲ/Large animal experiments/Safety/Efficacy引用本文复制引用
基金项目
国家自然科学基金(82370375)
陕西省重点研发计划(2024SF-YBXM-428)
陕西省重点研发计划(2023-YBSF-105)
空军军医大学校级项目(KJ2023C0KYD31)
出版年
2024
NETL
NSTL
万方数据