Immunoregulation of dendritic cells by lentinan in mice infected with Plasmodium yoelii
Objective To observe the immunoregulation of dendritic cells by lentinan in mice infected with Plasmodium yoelii. Methods A total of 120 female BALB/c mice were inoculated with red blood cells infected with 1×106 Py17XL P. yoelii and were randomly divided into an experimental group and control group. The experimental group was intraper-itoneally injected with lentinan, and the control group was injected with PBS. The average survival time of mice and parasite levels of viremia were determined, and the levels of IL-2, IL-10, IL-12, TGF-β, CD86, and TLR-9 were detected in mouse spleen DCs using Western blotting. Results The average survival time was (8. 35±2. 55) days for the experimental group and (5. 30+1.26) days for the control group; the difference was significant (P<0. 05). The experimental group had higher levels of expression of IL-2, IL-12, TGF-β, CD86, and TLR-9 in spleen DCs than did the control group; the difference was significant (P<0. 05). The experimental group had higher levels of IL-10 expression than did the control group; the difference was significant (P<0. 05). Conclusions Lentinan acts against malaria, furthering the maturation and enhancing the antigen-presenting role of dendritic cells by up-regulating the levels of IL-2, IL-12, TGF-β, CD86 and TLR-9.
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