Investigation of Anisakis larvae infection in marine fishes from Shanghai market
To understand the Anisakis larvae infection status in marine fishes sold in Shanghai,the fresh ma-rine fishes caught in the East China Sea area were collected from Farmers'markets,supermarkets and seafood mar-kets in Shanghai in 2022.The suspected Anisakis larvae were searched in the offal and muscles after dissection and observed under optical microscope and scanning electron microscope,respectively.A total of 338 marine fish of 16 species were collected,and 1 065 Anisakis larvae were found from 116 fish of 6 species,with a total infection rate of 34.3%(116/338)and average infection intensity of 9.2 larvae/fish.The highest infection rate was 11/12 in Lophiiformes,and the highest average infection intensity was 13.0 larvae/fish in Larimichthys polyactis.The Anisakis larvae infection rates increased gradually from spring to winter.The infection rate and average infection intensity in winter were 51.1%(46/90)and 12.3 larvae/fish,respectively,which were the highest seasons of the year.The predominant sites of Anisakis larvae parasitise in marine fishes were the intestines and abdominal cavity,with infection rates of 54.6%(582/1 065)and 40.7%(433/1 065),respectively.The result showed that Anisakis larvae infections were present in marine fish sold in Shanghai and the infection rates of commonly consumed marine fishes such as Lophiiformes and L.polyactis were high.