蚤亚科线粒体基因组研究进展
Research advances on Pulicinae mitochondrial genomes
林小霞 1董文鸽1
作者信息
- 1. 大理大学病原与媒介生物研究所,云南省自然疫源性疾病防控技术重点实验室,云南大理 671000
- 折叠
摘要
蚤亚科(Pulicinae)隶属于蚤目(Siphonaptera)蚤总科(Pulicoidea)蚤科(Pulicidae),是啮齿动物和猫犬常见的嗜血性体外寄生虫.已测定的蚤亚科共3属5种(猫栉首蚤、犬栉首蚤、东洋栉首蚤、印鼠客蚤和人蚤),其线粒体基因组的结构特征和变异情况具有以下特点:线粒体基因组保留了节肢动物典型的排列模式,未发生基因缺失、增加和重排现象;AT含量较高,且AT-偏斜和GC-偏斜均为负值,与大多数全变态类昆虫一样,密码子使用度具有明显的偏好性;人蚤的丝氨酸转运RNA(trnS1)呈典型的三叶草结构,其余物种与后生动物一样trnS1缺失D-臂,且5种蚤亚科物种的trnS1以UCU为反密码子代替了常见的GCU;蚤亚科控制区偏大,最大可达7 kb以上,主要以二核苷酸重复为主,NADH脱氢酶亚基4(nad4)～nad4L之间存在7 bp的保守重叠区,与多数全变态类昆虫一致.本文以期对今后蚤亚科在全变态类昆虫中的演化提供参考.
Abstract
Pulicinae belongs to Siphonaptera,Pulicoidea,Pulicidae,are common haematophagous ectoparasites in rodents,cats and dogs.A total of 3 genera and 5 species(Ctenocephalides felis,C.canis,C.orientis,Xenopsylla cheopis and Pulex irritans)have been determined.The structural characteristics and polymorphisms of the mitogenome have the following characteristics:the mitogenome retains the typical arrangement of the arthropod ancestor without gene deletions,additions,or rearrangements;higher AT content and both AT-skewness and GC-skewness were negative.The preferred codon usage is similar to most holometabolous insects.The tRNA-Ser(trnS1)gene of P.irritans shows a typical cloverleaf structure,and the other species have the same trnS1 but lacks the D-arm with the metazoan ani-mals.The trnS1 of 5 species within the Pulicinae subfamily replaced the common GCU with UCU as the anticodon.The control region of the Pulicinae subfamily is relatively large with up to more than 7 kb in length and are mainly dinucleotide repeats.Similar to most holometabolous insects,there is a 7 bp conserved overlapping region between NADH dehydrogenase subunit 4(nad4)and nad4L.This article aims to provide reference for the evolution of the flea subfamily in holometamorphosis insects in the future.
关键词
蚤亚科/线粒体基因组/系统发育/分子进化Key words
Pulicinae/Mitochondrial genome/Phylogenetic/Molecular evolution引用本文复制引用
出版年
2024
NETL
NSTL
万方数据