《疟原虫核酸检测多重PCR法》标准解读
Interpretation of the Criteria for Detection of Malaria Parasite Nucleic Acid by Multiplex PCR Methods
王真瑜 1江莉 1余晴 1张耀光 1吴寰宇 1陈健 1朱民1
作者信息
- 1. 上海市疾病预防控制中心,上海 200336
- 折叠
摘要
遵循《团体标准化第1部分:良好行为指南》(GB/T 20004.1-2016)相关规定,按照《标准化工作导则》(GB/T 1.1-2009)体例编制《疟原虫核酸检测多重PCR法》(T/SPMA 004-2023).标准由7章组成,包括"适用范围""规范性引用文件""术语和定义""仪器设备""试剂材料""检测步骤"和"废弃物的处理和防止污染的措施",另附有1个资料性附录(技术原理)和4个规范性附录(2个检测方法、试剂配制、样本准备).该标准已由上海市预防医学会发布公告(沪预医会[2023]34号)实施,为各级疾病预防控制机构和医疗机构规范操作、提高样本检测质量提供了技术依据,弥补了《疟疾的诊断》(WS259-2015)核酸检测尚无细化操作步骤之不足.
Abstract
The Criteria for Detection of Malaria Parasite Nucleic Acid by Multiplex PCR Methods(T/SPMA 004-2023)(referred to as the Criteria)was compiled following the Social Organization Standardization-Part 1:Guide-lines for Good Practice(GB/T 20004.1-2016)and Standardization Working Guidelines(GB/T 1.1-2009).The Criteria is composed of seven chapters,including"the range of application""normative reference documents""terms and defini-tions""instruments and equipment""reagent and materials""detection steps"and"waste disposal and pollution pre-vention measures".One informative appendix(technical principle)and four normative appendices(two detection methods,reagent preparation and sample preparation).The Criteria was issued by Shanghai Preventive Medicine Association through No.34 of SJPM in 2023.The Criteria provides for the technical reference for normative operations and the quality of sample testing in disease control institutions and medical institutions.And the Criteria makes up for the de-ficiency of specific operating steps in nucleic acid detection of Criteria for Diagnosis of Malaria(WS 259-2015).
关键词
疟原虫/核酸/PCR/标准Key words
Plasmodium/Nucleic acid/PCR/Criteria引用本文复制引用
基金项目
第6轮上海市加强公共卫生体系建设三年行动计划(2023-2025)(GWⅥ-11.2-XD04)
第6轮上海市加强公共卫生体系建设三年行动计划(2023-2025)(GWⅥ-11.1-01)
第6轮上海市加强公共卫生体系建设三年行动计划(2023-2025)(GWⅥ-11.1-13)
上海市科技创新行动计划(20DZ2200300)
出版年
2024
NETL
NSTL
万方数据