TGF-β1在膝关节骨性关节炎患者中的表达
Expression of TGF-β1 in serum and local tissues of knee osteoarthritis
王聪聪 1魏文涛 2邵泓鑫 2王加英 1徐云1
作者信息
- 1. 潍坊市人民医院关节外科,山东潍坊 261041
- 2. 潍坊市第二人民医院,山东潍坊 261041
- 折叠
摘要
[目的]比较膝关节骨性关节炎(knee osteoarthritis,KOA)与非KOA患者中血清、膝关节软骨和软骨下骨TGF-β1(transforming growth factor-β1)的表达.[方法]30例行人工全膝关节置换的KOA患者(KOA组),另30例非KOA患者,包括13例前交叉韧带急性损伤行韧带重建及髁间窝成形术的患者为对照组.取血清和膝关节软骨、软骨下骨检测TGF-β1.[结果]ELISA法检测患者血清中TGF-β1结果,KOA组(1 929.1±76.8)pg/ml,对照组为(1 611.4±105.9)pg/ml,差异存在统计学意义(P=0.022).软骨与骨组织TGF-β1免疫组织化学染色显示:KOA组强阳性24例,阴性6例,阳性率为80.0％;对照组阳性4例,阴性9,阳性率为30.8％.KOA组患者膝关节组织中的TGF-β1免疫组织化学染色的阳性率高于对照组,差异存在统计学意义(P=0.002).[结论]与非KOA的患者相比,KOA患者的血清和膝关节相关组织中TGF-β1表达显著升高.
Abstract
[Objective]To compare the expression of transforming growth factor-β1(TGF-β1)in serum and in knee cartilage and sub-chondral bone in knee osteoarthritis(KOA)and non-KOA patients.[Methods]Thirty patients with KOA who underwent total knee replace-ment(the KOA group)and another 30 patients without KOA,including 13 patients with acute anterior cruciate ligament injury who under-went ligament reconstruction and intercondylar fossaplasty(the control group)had TGF-β1 detected in serum,knee cartilage and subchon-dral bone.[Results]The results of TGF-β1 in serum by ELISA were(1 929.1±76.8)pg/ml in the KOA group,while(1 611.4±105.9)pg/ml in the control group,with a statistically significant difference(P=0.022).Immunohistochemical staining of TGF-β1 in cartilage and bone tis-sues showed that there were 24 strong positive cases and 6 negative cases in KOA group with a positive rate of 80.0％,whereas 4 positive cas-es and 9 negative cases with the positive rate of 30.8％in the control group,and the positive rate of TGF-β1 immunohistochemical staining in KOA group was higher than that in control group(P=0.002).[Conclusion]Compared with non-KOA patients,TGF-β1 expression in se-rum and knee joint related tissues was significantly increased in the KOA patients.
关键词
膝关节骨性关节炎/TGF-β1/软骨/软骨下骨Key words
knee osteoarthritis/transforming growth factor-β1(TGF-β1)/cartilage/subchondral bone引用本文复制引用
基金项目
山东省医药卫生科技发展计划项目(202004070094)
华通国康医学科研专项项目(2023HT050)
山东省医药卫生科技发展计划项目(202204070236)
出版年
2024
NETL
NSTL
万方数据