[Objective]To observe and detect the osteogenic capacity of allogenic cortical bone anchors implanted into the humeral head in mice.[Methods]A total of 54 C57BL6/J mice were randomly divided into two groups,27 mice were used for preparing allogenic cor-tical bone anchors,while another 27 mice were used as receptors for allograft cortical bone anchors.Three animals were sacrificed for tissue observation and immunohistochemical detection at 1 week,2 weeks,4 weeks,6 weeks,8 weeks and 10 weeks after implantation,additional-ly,for Mcro-CT at 1,4 and 10 weeks respectively.[Results]In term of histological observation,trabecular bone gradually increased around allogenic cortical bone anchors after implantation,while the allogenic cortical bone anchor was gradually absorbed,with new bone growing into the interior of allogenic cortical bone anchors.Micro-CT showed that the trabecular volume increased significantly with the time of 1,4 and 10 weeks[(0.20±0.37)mm3,(0.27±0.35)mm3,(0.34±0.38)mm3,P=0.001].Immunohistochemical assays showed that with time of 1,2,4,6,8 and 10 weeks after implantation,osteocalcin(OCN)[(0.48±0.05),(0.65±0.05),(0.64±0.06),(0.68±0.11),(0.73±.03),(0.72±0.03),P=0.004],TGF-β1[(0.49±0.02),(0.58±0.02),(0.64±0.02),(0.67±0.01),(0.72±0.01),(1.07±0.07),P＜0.001],andc-Jun N-terminal kinase(1JNK1)[(0.51±0.02),(0.63±0.01),(0.65±0.01),(0.68±0.07),(0.71±0.10),(0.83±0.19),P=0.022]significantly increased in term of optical density(OD)values.[Conclusion]The biological properties of mouse allogenic cortical bone anchors can be verified by implantation into hu-merus head,and the osteogenic capacity of the allogenic cortical bone anchors in host bone is proved to be good.
mousebone anchorin vivo implantationhumeral headosteogenic capacity
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