Bone marrow stem cells composited with polylactic glycolic acid frame for repairing cartilage defect in rats
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[目的]探索骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)与纳米聚乳酸羟基乙酸(polyaiticgly-colic Acid,PLGA)复合物修复大鼠股骨软骨缺损的效果.[方法]从大鼠胫骨和股骨骨髓腔内获取BMSCs并培养传代,之后培养到纳米PLGA支架材料上,进行电镜下观察.将SD大鼠的双后肢进行手术制造股骨远端软骨缺损,右侧缺损填充BMSC-PLGA复合材料(移植组),而左侧缺损不再做任何处理(空白对照组).于术后1个月和3个月进行大体观察评分和组织学评分,测定并比较两组甲苯胺蓝染色和免疫组化染色光密度值.[结果]BMSCs呈特有的螺旋状生长,PLGA电镜下呈纤维交织网状结构,BMSCs在材料上生长良好.植入体内后1、3个月移植组大体观察评分[(8.1±0.8)vs(1.7±0.8),P＜0.001;(10.3±1.2)vs(3.8±1.5),P＜0.001]、组织学评分[(11.6±1.0)vs(4.0±0.9),P＜0.001;(15.5±1.0)vs(4.8±0.8),P＜0.00l]、甲苯胺蓝染色 OD 值[(0.2±0.0)vs(0.l±0.0),P＜0.001;(0.4±0.0)vs(0.1±0.0),P＜0.001],术后 3 个月免疫组化 Ⅱ 胶原检测 OD 值[(0.4±0.0)vs(0.1±0.0),P＜0.001 均显著优于空白组.[结论]LGA结合BMSCs有效修复了大鼠软骨缺损,为软骨缺损治疗展示了可能的组织工程修改方法.
[Objective]To investigate the effect of bone marrow mesenchymal stem cells(BMSCs)composied with nano-polyaiticglycol-ic acid(PLGA)frame on repairing femoral cartilage defect in rats.[Methods]BMSCs were obtained from the bone marrow cavity of the tibia and femur of rats,cultured and passed through,and then cultured on the nano-PLGA scaffold frame,and the graft was observed under elec-tron microscope.Eighteen SD rats were operated on both hind limbs to create cartilage defect on the distal femur,and the defect on the right side was filled with BMSC-PLGA composited material(the graft group),whereas the defect on the left side left not further treated[the blank control(BC)group].Gross and histological observation and scoring,as well as optical density values measured in toluidine blue staining and immunohistochemical staining were compared between the two groups.[Results]BMSCs grew in a unique spiral shape,the PLGA frame showed fiber interwoven network structure under electron microscope,and BMSCs grew well on the PLGA frame.At 1 and 3 months after implantation,the graft group proved significantly superior to the BC group in terms of gross observation scores[(8.1±0.8)vs(1.7±0.8),P＜0.001;(10.3±1.2)vs(3.8±1.5),P＜0.001],histological score[(11.6±1.0)vs(4.0±0.9),P＜0.001;(15.5±1.0)vs(4.8±0.8),P＜0.001],toluidine blue staining OD value[(0.2±0.0)vs(0.1±0.0),P＜0.001;(0.4±0.0)vs(0.1±0.0),P＜0.001],in addition,immunohistochemical Ⅱ collagen de-tection OD value[(0.4±0.0)vs(0.1±0.0),P＜0.001]3 months postoperatively.[Conclusion]BMSCs-PLGA composition does effectively re-pair cartilage defects in rats in this study,demonstrating a potential tissue engineering modification method for the treatment of cartilage de-fects.
bone marrow mesenchymal stem cells(BMSCs)polyaiticglycolic acid(PLGA)ratscartilage defecttissue engineering
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