二代测序在假体周围感染诊断应用的进展
Progress of second generation sequencing in the diagnosis of periprosthetic joint infection
刘永飞 1张杰 1曹建泽 1赵海燕1
作者信息
- 1. 兰州大学第一临床医学院,甘肃兰州 730000;兰州大学第一医院骨科,甘肃兰州 730000
- 折叠
摘要
假体周围感染是关节置换术后的一种严重并发症,需要大量的医疗资源,给患者和社会带来了巨大的经济负担.近年来,随着新型分子诊断技术的不断发展,假体周围感染的诊断和治疗取得了重大进展.与传统培养相比,二代测序可以快速、准确、全面地诊断致病微生物,提高假体周围感染中致病微生物的检出率,从而实现早期诊断和治疗假体周围感染.本文综述了二代测序的原理、方法和其在假体周围感染中识别致病微生物的特点、优势和局限性.
Abstract
Periprosthetic joint infection(PJI)is a serious complication after joint replacement,which requires a lot of medical resources and brings a huge economic burden to patients and society.In recent years,with the continuous development of novel molecular diagnostic techniques,the diagnosis and treatment of PJI have made great progress.Compared with traditional culture,the next-generation sequencing can diagnose pathogenic microorganisms quickly,accurately and comprehensively,and improve the detection rate of pathogenic microorgan-isms in PJI,so as to achieve early diagnosis and treatment of PJI.This paper reviews the principle and method of next-generation sequencing and its characteristics,advantages and limitations in identifying pathogenic microorganisms in PJI.
关键词
二代测序/假体周围感染/诊断Key words
next generation sequencing/periprosthetic joint infection/diagnosis引用本文复制引用
基金项目
国家自然科学基金项目(82060394)
兰州市人才创新创业项目(2020-RC-45)
兰州大学第一医院院内基金项目(ldyyyn2022-73)
出版年
2024
NETL
NSTL
万方数据