摘要
[目的]探讨蛇葡萄根提取物对慢性骨髓炎反应的影响.[方法]将大鼠分为5组,分别为正常组、模型组、低剂量、中剂量和高剂量.每组6只.模型组、低剂量、中剂量和高剂量大鼠接受骨髓炎造模手术.正常组和模型组大鼠每天灌服1次蒸馏水(10 ml);低剂量、中剂量、高剂量组大鼠每天灌服1次含蛇葡萄根提取物250μg/ml、500 μg/ml、1 000 μg/ml.给药8周后处死大鼠,检测NF-κB信号通路关键基因和炎症因子表达水平,X线片评价大鼠骨髓炎疾病严重程度.[结果]RT-qPCR检测,与正常组比较,模型组IL-6、IL-1β、TNF-αmRNA水显著增高(P＜0.05);与模型组比较,给药的三组上述指标均显著降低(P＜0.05).与正常组比较,模型组NF-κB、IKK、PU1、FOL水平显著增高(P＜0.05),IκB水平显著降低(P＜0.05);与模型组相比,低剂量、中剂量和高剂量 NF-κB[(1.8±0.0)vs(1.7±0.1)vs(1.4±0.1)vs(1.3±0.1),P＜0.001],IKK[(2.3±0.1)vs(2.0±0.1)vs(1.6±0.1)vs(1.3±0.0),P＜0.001]、PU1[(2.6±0.1)vs(1.9±0.1)vs(1.7±0.1)vs(1.4±0.1),P＜0.001]、FOLR[(2.1±0.1)vs(1.8±0.1)vs(1.5±0.1)vs(1.4±0.1),P＜0.001]水平指标均显著降低,而 IκB 水平[(0.4±0.0)vs(0.5±0.1)vs(0.6±0.1)vs(0.8±0.0),P＜0.001]显著升高,并呈剂量依赖性.Western blot检测,与正常组比较,模型组IL-6、IL-1β、TNF-α水平显著增高(P＜0.05),与模型组比较,三个给药组上述指标均显著降低(P＜0.05).[结论]蛇葡萄根提取物可以抑制NF-κB信号通路关键基因和炎症因子表达,降低炎性细胞浸润,对大鼠骨髓炎具有一定治疗作用.
Abstract
[Objective]To investigate the effect of radix smpelopsis sinicae extract on the healing process of chronic osteomyelitis in rat.[Methods]The rats were divided into 5 groups,including normal group,model group,low dose,medium dose and high dose,with 6 animals in each group.Of them,the rats in model group,low dose,medium dose and high dose rats underwent osteomyelitis model making operation.Normal group and model group were given distilled water(10 ml)once a day,whereas low dose,medium dose and high dose groups were giv-en 250 μg/ml,500 μg/ml and 1 000 μg/ml of radix smpelopsis sinicae extract respectively once a day.After 8 weeks of administration,the rats were killed,and the expression levels of key genes and inflammatory factors in NF-κB signaling pathway were detected,while the severi-ty of osteomyelitis was evaluated by X-ray and histology.[Results]As for RT-qPCR assay,the model group had significant increases in mRNA levels of IL-6,IL-1 β and TNF-α compared with the normal group(P＜0.05);however,the three drug-given groups got significantly decreases regarding abovesaid items compared with the model group(P＜0.05).In addition,the model group had significant increases in the levels of NF-κB,IKK,PU1 and FOL,while significant decline of IκB compared with the normal group(P＜0.05).Compared with the model group,the low dose,middle dose and high dose groups got significant decrease in terms the nf-kappa B[(1.8±0.0)vs(1.7±0.1)vs(1.4±0.1)vs(1.3±0.1),P＜0.001].IKK[(2.3±0.1)vs(2.0±0.1)vs(1.6±0.1)vs(1.3±0.0),P＜0.001],PU1[(2.6±0.1)vs(1.9±0.1)vs(1.7±0.1)vs(1.4±0.1),P＜0.001],FOLR[(2.1±0.1)vs(1.8±0.1)vs(1.5±0.1)vs(1.4±0.1),P＜0.001],whereas significant upgrading in IκB level[(0.4±0.0)vs(0.5±0.1)vs(0.6±0.1)vs(0.8±0.0),P＜0.001]in dosage-dependent manner.Regarding western blot analysis,the model group had significant increase in IL-6,IL-1β and TNF-α compared with the normal group(P＜0.05),while the above indexes were significantly decreased in the three drug administration groups compared with the model group(P＜0.05).[Conclusion]The radix smpelopsis sinicaeextract might inhibit the expres-sion of key genes of NF-κB signaling pathway and inflammatory factors,reduce inflammatory cell infiltration,and has a certain therapeutic effect on osteomyelitis in rats.
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