Immunology is a basic courses in medical education,and a compulsory main course for medical college students.It has strong logic,abstract content and great teaching difficulty.In the past,many teaching modes of immunology were teacher centered,with teachers speaking and students listening.It was difficult to fully mobilize students'learning initiative,which made many students afraid of the course and had no interest in it.Students need online learning,how to develop online intelligence teaching,improve learner autonomy and ensure learning effectiveness.In recent years,with the introduction of PBL teaching method,many immunology teachers have also carried out relevant teaching reform research.With the smart environment built by rain classroom and Tencent conference,the application of PBL teaching method becomes more and more flexible.This paper systematically evaluates the application of PBL(Problem-Based Learning)teaching method based on rain classroom and Tencent conference in immunology courses,to explore its advantages and limitations,and deeply explore the significance of this teaching method for immunology teaching.