Molecular Biology is a new frontier subject which studies the essence of life at the molecular level.It is a bridge course connecting basic medicine and clinical medicine,normal physiological state and disease state.It is an important frontier field of life science,which is developing fastest and is crossing with other subjects widely.It is not only an important basic medical course,but also a cross course closely related to clinic.Its teaching reform has always been one of the important contents of basic medical education reform in medical colleges and universities.In response to the call of carrying out the first-class undergraduate curriculum construction in an all-round way,with the purpose of building the offline first-class undergraduate course of Molecular Biology,this paper will explore the teaching reform of Molecular Biology course from six aspects:course introduction,course status and main existing problems,curriculum reform objectives,teaching reform exploration and practice,the curriculum innovation and characteristics,and the effect and evaluation of course construction.It provides suggestions and reflections on the construction of the offline first-class undergraduate courses.