Objective To observe the effect of deep muscle stimulation on muscle structure and function in children with spastic ce-rebral palsy.Methods From January,2023 to March,2024,30 children with spastic cerebral palsy in Beijing Bo'ai Hospital were ran-domly divided into control group(n=15)and intervention group(n=15).Both groups received conventional re-habilitation therapy,while the intervention group added deep muscle stimulation,for four weeks.Before and after training,the gastrocnemius muscle was examined by ultrasound diagnosis system in two aspects:transverse inci-sion and longitudinal incision.Indicators included fascicle length,muscular thickness,cross sectional area and pennation angle.The modified Ashworth Scale(MAS)was used to assess the muscle tension of the lower extrem-ity of the affected side.The standing,walking,running and jumping function were evaluated by Gross Motor Function Measure(GMFM)D and E areas.Results After training,the fascicule length and pennation angle improved significantly in both groups(|t|>6.329,P<0.001),and they were better in the intervention group than in the control group(|t|>2.347,P<0.05);the scores of MAS decreased in both groups(t>2.432,P<0.05),and were better in the intervention group than in the con-trol group(t=2.140,P<0.05);the scores of GMFM D and E areas significantly decreased in both groups(|t|>8.473,P<0.001),and were better in the intervention group than in the control group(|t|>2.191,P<0.05).Conclusion Deep muscle stimulation could improve the fascicule length and pennation angle,and improve the spasticity and motor function of lower extremities in children with spastic cerebral palsy.