慢性乙型肝炎患者血清HBsAg与单个核细胞乙型肝炎病毒RNA水平对聚乙二醇干扰素治疗效果的预测价值
Utility of serum HBsAg and peripheral blood mononuclear cell HBV RNA in predicting the treatment effect of pegylated interferon α-2b in patients with chronic hepatitis B
席文娜 1罗飞兵 2吴昭 3余东山1
作者信息
- 1. 南昌大学第二附属医院感染科,南昌 330006
- 2. 丰城市人民医院感染科
- 3. 南昌大学第二附属医院检验科,南昌 330006
- 折叠
摘要
目的 探讨慢性乙型肝炎患者外周血清HBsAg、乙型肝炎病毒(HBV)DNA与血清HBV RNA及单个核细胞(PBMC)HBV RNA的关系.方法 50 例慢性乙型肝炎患者,给予Peg-IFNα-2b 180 μg皮下注射,每周一次,分别在初始治疗、24周和 48 周,检测患者血清HBV五项、肝功能、HBV DNA、HBV RNA及PBMC中HBV RNA的变化.结果 患者三个时间段的生化指标ALT、AST、TBIL、AKP及GGT比较差异无统计学意义(P＞0.05);免疫学标志物HBsAg、HBsAb、HBeAg、HBeAb差异有统计学意义(P＜0.05);血清HBV DNA及HBV RNA与PBMC HBV RNA差异有显著统计学意义(P＜0.001).结论 Peg-IFNα-2b抗病毒治疗各时间段,肝功能转氨酶无显著变化;治疗 24 周,血HBsAg、HBV DNA与HBV RNA及PBMC HBV RNA快速下降,有显著相关性;治疗48周,血清HBsAg、HBV DNA与HBV RNA及PBMC HBV RNA相关性减弱.因此,24周HBV RNA下降幅度优于48周,更能预测临床治愈.
Abstract
Objective To investigate the relationship between serum HBV markers(HBsAg,HBV DNA and HBV RNA)and peripheral blood mononuclear cell(PBMC)HBV RNA in patients with chronic hepatitis B(CHB)receiving pegylated interferon α-2b(Peg-IFN α-2b).Methods A total of 50 CHB patients received Peg-IFNα-2b 180 μg weekly by subcutaneous injection.Serum markers of hepatitis B including HBV DNA and HBV RNA,liver function tests,and PBMC HBV RNA were evaluated before treatment,and after treatment for 24 and 48 weeks,respectively.Results The liver function tests including alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bilirubin(TBIL),alkaline phosphatase(AKP),and gamma-glutamyl transpeptidase(GGT)did not show significant differences after treatment from 0 to 48 weeks(P>0.05).The serum markers HBsAg,HBsAb,HBeAg and HBeAb showed significant changes after treatment(P<0.05).Significant change was observed for serum HBV DNA,HBV RNA and PBMC HBV RNA from 0 to 48 weeks of treatment(P<0.001).Conclusions Liver function tests did not show significant change during Peg-IFNα-2b treatment for 48 weeks.Serum HBsAg,HBV DNA,HBV RNA,and PBMC HBV RNA decreased rapidly after treatment for 24 weeks.Improvement of these markers were correlated significantly with Peg-IFNα-2b treatment.The intensity of the correlation decreased after treatment for 48 weeks.Therefore,the decrease of HBV RNA after 24-week treatment is a better indicator predictive of clinical cure than the reduction of HBV RNA after 48-week treatment.
关键词
乙型肝炎病毒RNA/单个核细胞乙型肝炎病毒RNA/Peg-IFNα-2b/慢性乙型肝炎Key words
HBV RNA/peripheral blood mononuclear cell/pegylated interferon α-2b/treatment effect/chronic hepatitis B引用本文复制引用
基金项目
江西省卫生健康委科技项目(20204250)
江西省卫生健康委科技项目(20204246)
出版年
2024
NETL
NSTL
维普
万方数据