PURPOSE:To investigate the effect of Sophora japonica extract on osteogenic differentiation of jaw bone marrow mesenchymal stem cells(jBMSCs).METHODS:The effects of 10,25,50 and 100 μmol/L Sophora japonica ex-tract solutions on proliferation of jBMSCs were detected by CCK-8.BCIP/NBT alkaline phosphatase and alizarin red staining were used to detect the effect of 10,25,50 μmol/L extracts of Sophora on osteogenic differentiation of jBMSCs.Western blot analysis and real-time quantitative PCR were used to detect the effects of Sophora japonica extract solution on protein and mRNA expression levels of osteogenic differentiation-related genes of jBMSCs.GraphPad Prism 9 software package was used for data analysis.RESULTS:The experimental results showed that 100 μmol/L Sophora japonica ex-tract solution had obvious toxic effect on jBMSCs(P＜0.0001);while 10,25 and 50 μmol/L Sophora japonica extract solu-tion did not affect cell proliferation(P＞0.05).Compared with the control group,alkaline phosphatase activity,number of calcium nodules,and protein and mRNA expression levels of osteogenic differentiation-related molecules in the 10,25 and 50 μmol/L groups were gradually increased.CONCLUSIONS:The extract of Sophora japonica on 10,25,50 μmol/L can enhance the osteogenic differentiation of jBMSCs.
关键词
槐角提取物/颌骨骨髓间充质干细胞/成骨向分化/细胞增殖
Key words
Sophora japonica extract/Jaw bone marrow mesenchymal stem cells/Osteogenic differentiation/Cell proliferation
维普
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