PURPOSE:To investigate the effects of Lycorine,a natural plant extract,on oral squamous cell carcinoma(OSCC)cells and its underlying mechanisms.METHODS:Plate cloning assay and flow cytometry were used to evaluate the inhibitory effect of Lycorine on the proliferation of OSCC in vitro.Scratch assay and Transwell assay were conducted to assess the impact of Lycorine on the invasion of OSCC.Western blot and immunofluorescence were performed to confirm the inhibitory effect of Lycorine on SCAP protein in OSCC.A nude mouse xenograft model was established to evaluate the in vivo anticancer effect of Lycorine against oral squamous cell carcinoma.Graphpad Prism software package was used for statistical analysis.RESULTS:Plate cloning assay and flow cytometry showed that Lycorine significantly inhibited the proliferation of OSCC cells in vitro.Scratch assay and Transwell assay revealed a significant inhibitory effect of Lycorine on the migration and invasion of OSCC cells.Western blot and immunofluorescence results demonstrated that Lycorine could degrade SCAP protein in OSCC cells,affecting cholesterol metabolism in cancer cells.Animal experiment results further indicated that Lycorine exhibited potent anticancer activity in vivo,significantly suppressing the growth of OSCC cells and reducing the levels of SCAP protein within cancer cells.CONCLUSIONS:Lycorine can suppress the prolifera-tion and invasion of OSCC cells by degrading SCAP protein,making it a potential low-toxicity and highly effective natural plant extract for the prevention and treatment of OSCC.
维普
万方数据