PURPOSE:Liposomes were used to simultaneously encapsulate basic fibroblast growth factor(bFGF)that can promote nerve differentiation and osteogenic small molecule(Dex).Then,a temperature sensitive and injectable bFGF/Dex liposome-hydrogel composite sustained-release scaffold was constructed by mixing it with sodium alginate solution.METHODS:bFGF/Dex liposomes were prepared by film dispersion method,mixed with sodium alginate solution activated by RGD polypeptide,and thermosensitive bFGF/Dex liposome hydrogel composite scaffolds were synthesized by free radi-cal polymerization.The particle size,polydispersity,and Zeta potential of liposomes were detected by Zeta particle size analyzer.Microstructure of the scaffold was observed under scanning electron microscopy,and mechanical strength of the scaffold was measured using a universal material testing machine.Real time fluorescence quantitative PCR(RT-PCR)and immunofluorescence staining were used to detect the expression of bFGF promoting neural differentiation related genes and proteins in hBMSCs.Rabbit skull defect models were constructed and the effect of the composite scaffold on promoting neurogenic bone regeneration in vivo was evaluated through micro-CT and immunohistochemical staining.SPSS 26.0 soft-ware package was used for statistical analysis.RESULTS:Thermosensitive bFGF/Dex liposome hydrogel composite scaf-fold was successfully constructed.It was in a solution state and had fluidity at normal temperature(25℃),and quickly changed to a gel state when higher than the critical temperature(32 ℃).The surface of scaffold was loose and porous,which could bear certain stress.The expression of genes and proteins related to neurogenesis suggested that bFGF had a strong promoting effect on neurogenesis in hBMSCs.The results of animal experiments showed that the composite had a good neurogenic bone regeneration effect.CONCLUSIONS:The intelligent bFGF/Dex liposome hydrogel composite scaffold with temperature sensitivity and outstanding biological function provides a new material for repair of jaw defects.
关键词
颌骨缺损/骨移植材料/神经化骨再生/温敏性复合支架
Key words
Jaw bone defect/Bone graft materials/Neurogenic bone regeneration/Temperature sensitivity composite scaffold
维普
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