BMP-2联合雷奈酸锶对大鼠骨髓间充质干细胞成骨分化的影响
Effect of BMP-2 combined with strontium ranelate on osteogenic differentiation of rat bone marrow mesenchy-mal stem cells
袁润林 1李亚明2
作者信息
- 1. 乐山老年病专科医院 口腔科门诊,四川 乐山 614000
- 2. 成都市第二人民医院 口腔科,四川 成都 610000
- 折叠
摘要
目的:研究骨形态发生蛋白2(bone morphogenetic protein-2,BMP-2)联合雷奈酸锶(strontium ranelate,Sr)对大鼠骨髓间充质干细胞(bone mesenchymal stem cells,BMSCs)成骨分化的影响,并探讨其相关机制.方法:取对数期大鼠BMSCs,随机分为对照组(不采用任何诱导液处理)、BMP-2组(0.1 mg/L BMP处理)、Sr组(5 mmol/L Sr处理)、BMP-2+Sr组(采用0.1 mg/L BMP处理6天后换液,再加入5 mmol/L Sr处理).利用MTT实验检测BMSCs增殖情况,硝基苯磷酸盐法检测碱性磷酸酶(alkaline phosphatase,ALP)活性,茜素红染色观察矿化情况,Western免疫印迹法检测TGF-β1、Smad2、p-Smad2及Runx2蛋白表达.采用SPSS 24.0软件包对数据进行统计学分析.结果:与对照组相比,BMP-2组、Sr组、BMP-2+Sr组ALP活性增强,MTT实验吸光度值、矿化面积百分比及TGF-β1、Runx2蛋白表达量,p-Smad2/Smad2水平显著升高(P＜0.05).与BMP-2组、Sr组相比,BMP-2+Sr组ALP活性增强,MTT实验吸光度值、矿化面积百分比及TGF-β1、Runx2蛋白表达量,p-Smad2/Smad2水平显著升高(P＜0.05).结论:BMP-2联合Sr可促进大鼠BMSCs增殖及成骨分化,其作用机制可能与激活TGF-β1/Smad信号通路有关.
Abstract
PURPOSE:To study the effect of bone morphogenetic protein-2(BMP-2)combined with strontium ranelate(Sr)on osteogenic differentiation of rat bone mesenchymal stem cells(BMSCs),and explore the related mechanisms.METHODS:Log-phase BMSCs were taken and randomly divided into control group(without any induction solution treatment),BMP-2 group(0.1 mg/L BMP treatment),Sr group(5 mmol/L Sr treatment),BMP-2+Sr group(after treatment with 0.1 mg/L BMP for 6 days,changed the solution and added 5 mmol/L Sr for treatment).Cell proliferation was detected by MTT experiment.Alkaline phosphatase(ALP)activity was detected by nitrophenyl phosphate method.Mineralization was observed by alizarin red staining.Protein expression of transforming growth factor β1(TGF-β1),Smad2,p-Smad2,and Runt-related transcription factor 2(Runx2)were detected by Western blot.SPSS 24.0 software package was used for data analysis.RESULTS:Compared with the control group,the ALP activity of BMP-2 group,Sr group,and BMP-2+Sr group were enhanced,of which MTT experimental absorbance(A)value,the percentage of mineralized area and the expression of TGF-β1 and Runx2 protein,p-Smad2/Smad2 were significntly increased(P＜0.05).Compared with BMP-2 group and Sr group,the ALP activity of the BMP-2+Sr group were enhanced,of which MTT experimental A value,the percentage of mineralized area and the expression of TGF-β1 and Runx2 protein,p-Smad2/Smad2 were significantly increased(P＜0.05).CONCLUSIONS:BMP-2 combined with Sr can promote the proliferation and osteogenic differentiation of BMSCs in rats,the mechanism of which may be related to the activation of TGF-β1/Smad signaling pathway.
关键词
大鼠/骨髓间充质干细胞/成骨分化/骨形态发生蛋白2/雷奈酸锶Key words
Rat/Bone mesenchymal stem cells/Osteogenic differentiation/Bone morphogenetic protein-2/Strontium ranelate引用本文复制引用
出版年
2024
NETL
NSTL
万方数据