PURPOSE:To isolate and identify Capnocytophaga sputigena(C.sputigena)from tongue squamous cell carci-noma(TSCC),then analyze the drug resistance and virulence genes at genome-wide level.METHODS:The bacterial strains n105 and n106 were isolated and cultured from the cancer tissue mucosa of TSCC patients.Bacterial species were determined through colony morphology,Gram staining,16S rRNA sequence,and construction of evolutionary tree.Then the drug sensitivity of bacterial strains to 4 antibiotics(clarithromycin,metronidazole,penicillin and levofloxacin)was de-tected by disk diffusion method.Whole genome sequencing and functional annotation of virulence and resistance genes were performed on strain n106 by the third-generation sequencing technology.RESULTS:Strains n105 and n106 were identified as C.sputigena through 16S rRNA sequence BLAST alignment and construction of Neighbor Joining evolutionary trees.The results of drug sensitivity test showed that C.sputigena strains n105 and n106 were sensitive to clarithromycin,while resistant to metronidazole,penicillin,and levofloxacin.Whole genome sequencing results showed that the genome length of C.sputigena strain n106 was 2.86 Mb,with 38.36％of guanine cytosine(GC)content,encoding 2 609 genes.Six virulence genes(tufA,app,clpV,cpsA,acpXL and fHbp)and two drug resistance genes[blaCSP-land erm(F)]were pre-dicted.CONCLUSIONS:C.sputigena isolated from TSCC is resistant to metronidazole,penicillin,and levofloxacin,car-rying multiple virulence and resistance genes,which possessing potential pathogenic characteristics.This study provides new strategies for effective prevention,control,and treatment of TSCC from a microbial perspective.
万方数据
维普
