Enhanced production of tylosin fermentation by genome shuffling
Objective To improve the production oftylosin by genome shuffling.Methods Genome shuffling was applied to improve tylosin-producing by utilizing the four highest strains derived on scripture mutation as the starting population.A recombinant TLA16-336 with improved phenotype was obtained by three rounds of genome shuffling,and its production reached 15.62g/L,which was increased by 24.7％ and 32.9％ compared with that of the highest parent strain and the original strain,respectively.Results Fermentation experiments by TLA16-336 were conducted in 5L fermentor.The tylosin production of the highest producer mutant reached 16.03g/L,which was increased by 20.5％ compared with that of the original strain.Conclusion Genome rearrangement was feasible method for improving fermentation yield.
NETL
NSTL
万方数据
维普
