The secondary metabolites isolated from the marine-derived fungus Penicillium sp.and their antibacterial activities
Objective This study aimed to investigate the secondary metabolites from the marine-derived fungus Penicillium sp.LW23 and their antibacterial activities.Methods Silica gel column chromatography,Sephadex LH-20 gel column chromatography and reversed phase high-performance liquid chromatography(RP HPLC)were utilized to isolate and purify the fermented crude extract.Their structures were elucidated by NMR and MS analysis and comparison with the data from the literature;their antibacterial ativities in vitro were determined using the microbroth dilution technique.Results Six compounds were isolated and identified as methyl penicyrone(1),penicyrone(2),verrucosidin(3),deoxyverrucosidin(4),peniquinone A(5)and penipratynolene(6).Conclusion The marine-derived fungus Penicillium sp.LW23 could produce compounds with antibacterial activities.Compounds 3 and 4 showed moderate antibacterial activities against Helicobacter pylori G27 and triple-drug-resistant H.pylori BHKS159.While compound 5 showed certain antibacterial activities against Xanthomonas campestris pv.campestris,Ralstonia solanacearum,Bacillus subtilis and Mycobacterium smegmatis.
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