Isolation,nanoemulsion preparation and biological activity of pomegranate seed oil with different polarity
Objective The pomegranate seed oil(PSO)into fractions with different polarities was isolated to compare their biological activities.Methods PSO triglyceride(PSO TG)and PSO diglyceride(PSO DG)were isolated by silica gel column chromatography,respectively.Polyphenol-enriched PSO(P-PSO)was prepared by extracting PSO with anhydrous ethanol.The antioxidant activity of the oils was estimated by the radical-scavenging method.The antibacterial capacity was assessed using the filter paper approach.The four oils were formulated into nanoemulsions,and the inhibitory effects of nanoemulsions against α-glucoside and α-amylase were examined,respectively.Results The polarity order was P-PSO＞PSO-DG＞PSO＞PSO-TG.The scavenging rate against DPPH radicals was P-PSO＞PSO-TG＞PSO＞PSO-DG.PSO-DG had a significantly higher inhibitory effect than the other oils against Bacillus subtilis(P＜0.05),while PSO possessed the strongest antibacterial activity against yeast(Kazachstania exigua).The inhibitory strength of nanoemulsions on α-glucoside and α-amylase was PSO-DG＞PSO＞P-PSO＞PSO-TG.Conclusion The polarity of oil could influence its capacity to penetrate through the plasma membrane and cell wall of bacteria,as well as its affinity with enzymes.It is a key factor in the biological activity of oil.
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维普
