Objective This study aimed to investigate antimicrobial resistance in clinical isolates at Zigong First People's Hospital and provide a basis for the rational use of antimicrobial drugs.Methods Clinical isolates from our hospital between 2020 and 2022 were collected.The VITEK automated identification and drug sensitivity system,paper diffusion method,and E-test method were used for bacterial identification and drug sensitivity testing,and the results were judged by the 2022 American Clinical and Laboratory Standardization Institute(CLSI)fold point criteria.Results A total of 13324 clinical strains were isolated,of which Gram-negative bacteria accounted for 69.6％and Gram-positive bacteria accounted for 30.4％.The top five isolates were E.coli,K.pneumoniae,S.aureus,P.aeruginosa,and H.influenzae.The detection rates of methicillin-resistant S.aureus(MRSA)and methicillin-resistant coagulase-negative staphylococci(MRCNS)were 24.1％and 73.0％,respectively.The detection rates of linezolid-resistant E.faecium and E.faecalis were 2.1％and 12.4％of samples,respectively,and one vancomycin-resistant E.faecium strain was isolated.All four strains of S.pneumoniae isolated from cerebrospinal fluid were penicillin-non-susceptible strains,and no penicillin-resistant S.pneumoniae of non-cerebrospinal fluid origin were detected.The detection rate of carbapenem-resistant E.cloacae was 12.3％.The detection rates of carbapenem-resistant E.coli and K.pneumoniae were low,at 1.1％and 2.9％,respectively.The resistance rate of P.aeruginosa to piperacillin/tazobactam,cefepime,and gentamicin increased annually;the resistance rate of A.baumannii to common antibacterial drugs was significantly higher than that of P.aeruginosa;and the detection rates of carbapenem-resistant strains were 41.9％and 6.9％,respectively.Cefotaxime-resistant H.influenzae and M.catarrhalis were not isolated.Conclusion Clinical isolates were predominantly Gram-negative bacteria,and the resistance rates of common isolates showed a steady or slightly decreasing pattern.However,the detection rates of linezolid-resistant E.faecalis and amoxicillin/clavulanic acid-resistant H.influenzae increased significantly.Therefore,hospital infection prevention and control measures and the rational use of antimicrobial drugs should be strengthened.
关键词
细菌耐药监测/药物敏感试验/多重耐药菌/合理用药
Key words
Bacterial drug resistance monitoring/Drug sensitivity test/Multi-drug resistant bacteria/Rational drug use
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