Surveillance of antibiotic resistance in clinical isolates at Zigong First People's Hospital from 2020 to 2022
Objective This study aimed to investigate antimicrobial resistance in clinical isolates at Zigong First People's Hospital and provide a basis for the rational use of antimicrobial drugs.Methods Clinical isolates from our hospital between 2020 and 2022 were collected.The VITEK automated identification and drug sensitivity system,paper diffusion method,and E-test method were used for bacterial identification and drug sensitivity testing,and the results were judged by the 2022 American Clinical and Laboratory Standardization Institute(CLSI)fold point criteria.Results A total of 13324 clinical strains were isolated,of which Gram-negative bacteria accounted for 69.6％and Gram-positive bacteria accounted for 30.4％.The top five isolates were E.coli,K.pneumoniae,S.aureus,P.aeruginosa,and H.influenzae.The detection rates of methicillin-resistant S.aureus(MRSA)and methicillin-resistant coagulase-negative staphylococci(MRCNS)were 24.1％and 73.0％,respectively.The detection rates of linezolid-resistant E.faecium and E.faecalis were 2.1％and 12.4％of samples,respectively,and one vancomycin-resistant E.faecium strain was isolated.All four strains of S.pneumoniae isolated from cerebrospinal fluid were penicillin-non-susceptible strains,and no penicillin-resistant S.pneumoniae of non-cerebrospinal fluid origin were detected.The detection rate of carbapenem-resistant E.cloacae was 12.3％.The detection rates of carbapenem-resistant E.coli and K.pneumoniae were low,at 1.1％and 2.9％,respectively.The resistance rate of P.aeruginosa to piperacillin/tazobactam,cefepime,and gentamicin increased annually;the resistance rate of A.baumannii to common antibacterial drugs was significantly higher than that of P.aeruginosa;and the detection rates of carbapenem-resistant strains were 41.9％and 6.9％,respectively.Cefotaxime-resistant H.influenzae and M.catarrhalis were not isolated.Conclusion Clinical isolates were predominantly Gram-negative bacteria,and the resistance rates of common isolates showed a steady or slightly decreasing pattern.However,the detection rates of linezolid-resistant E.faecalis and amoxicillin/clavulanic acid-resistant H.influenzae increased significantly.Therefore,hospital infection prevention and control measures and the rational use of antimicrobial drugs should be strengthened.
Bacterial drug resistance monitoringDrug sensitivity testMulti-drug resistant bacteriaRational drug use
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