Quantitative proteomics analysis of a Salmonella enteritidis strain causing food poisoning incident
Objective Based on proteomics,the study was used to reveal the molecular genetic characteristics of a Salmonella enteritidis 21A that caused a food poisoning event,so as to better prevent and control the occurrence of foodborne diseases and ensure human life.Methods The quantitative proteomics technology was used to analyze Salmonella enteritidis 21 A,and the TimsTOF Pro instrument was used to collect mass spectrometry data in the data independent collection mode.The MSstats software was used to complete the quantitative analysis of peptides and proteins as well as the statistics of differential proteins,and the biological functions of the differentially expressed proteins were examined using the GO,KOG function enrichment,KEGG pathway enrichment analysis,CAZY annotation,and interaction analysis.Results The quantitative study of 21A revealed 3183 proteins,including 300 differential proteins.The GO database functional annotation indicated that differential proteins were primarily associated with catalytic processes,binding,intracellular processes,and metabolism,whereas the KOG and KEGG databases functional annotation demonstrated that difference proteins were mainly associated with six metabolic pathways.The CAZY database analyzed the carbohydrate-active enzymes in 21A,37.96%of which were glycoside hydrolases.Conclusion This work characterized the proteins of S.enteritidis 21 A,which used a number of survival tactics during the invasion and infection processes,including raising the production of virulence factors,promoting lipid breakdown,and driving iron acquisition.This in-depth examination of metabolism-related proteins may aid in the understanding of S.enteritidis invasion and infection mechanisms and better prevent and control the occurrence of foodbome illness.