首页|1株引起食物中毒事件的肠炎沙门菌定量蛋白质组学分析

1株引起食物中毒事件的肠炎沙门菌定量蛋白质组学分析

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目的 基于蛋白质组学,深入揭示1株引起食物中毒事件的肠炎沙门菌21A的分子特征,从而更好地防控食源性疾病的发生.方法 利用定量蛋白质组学技术对肠炎沙门菌株21A进行分析,使用TimsTOF Pro仪器在数据非依赖采集模式下采集质谱数据,利用MSstats软件完成肽段与蛋白的定量以及差异蛋白统计,并对差异表达蛋白的生物学功能进行GO、KOG功能富集、KEGG通路富集分析与CAZY注释、互作分析.结果 菌株21A中共鉴定出3183种蛋白质,其中差异蛋白300种.GO分析表明,差异蛋白主要与催化、结合、细胞内过程和代谢有关;KOG和KEGG分析显示,差异蛋白主要富集在6种代谢通路中;CAZY分析发现,37.96%的蛋白质为糖苷水解酶.结论 本研究揭示了肠炎沙门菌株21A的蛋白质组学特征,该菌株在入侵和感染过程中采用多种生存策略,包括增强毒力因子表达,增加脂质降解和诱导铁获取等.对代谢相关蛋白质的深入分析,有助于深入了解该菌的传播与感染机制,更好地防控食源性疾病的发生.
Quantitative proteomics analysis of a Salmonella enteritidis strain causing food poisoning incident
Objective Based on proteomics,the study was used to reveal the molecular genetic characteristics of a Salmonella enteritidis 21A that caused a food poisoning event,so as to better prevent and control the occurrence of foodborne diseases and ensure human life.Methods The quantitative proteomics technology was used to analyze Salmonella enteritidis 21 A,and the TimsTOF Pro instrument was used to collect mass spectrometry data in the data independent collection mode.The MSstats software was used to complete the quantitative analysis of peptides and proteins as well as the statistics of differential proteins,and the biological functions of the differentially expressed proteins were examined using the GO,KOG function enrichment,KEGG pathway enrichment analysis,CAZY annotation,and interaction analysis.Results The quantitative study of 21A revealed 3183 proteins,including 300 differential proteins.The GO database functional annotation indicated that differential proteins were primarily associated with catalytic processes,binding,intracellular processes,and metabolism,whereas the KOG and KEGG databases functional annotation demonstrated that difference proteins were mainly associated with six metabolic pathways.The CAZY database analyzed the carbohydrate-active enzymes in 21A,37.96%of which were glycoside hydrolases.Conclusion This work characterized the proteins of S.enteritidis 21 A,which used a number of survival tactics during the invasion and infection processes,including raising the production of virulence factors,promoting lipid breakdown,and driving iron acquisition.This in-depth examination of metabolism-related proteins may aid in the understanding of S.enteritidis invasion and infection mechanisms and better prevent and control the occurrence of foodbome illness.

Salmonella enteritidisProteomicsFood poisoningInfectionMetabolism

徐本锦、侯竹如、刘玲、严荣荣、张金晶、杜淼、宣焱、李卓禧、范蕾

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山西医科大学汾阳学院医学检验系,汾阳 032200

临床分子诊断学吕梁市重点实验室,汾阳 032200

山西省汾阳医院医学检验科,汾阳 032200

山西医科大学汾阳学院基础医学部,汾阳 032200

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肠炎沙门菌 蛋白质组学 食物中毒 感染 代谢

山西省基础研究计划(自由探索类)山西省基础研究计划(自由探索类)吕梁市引进高层次科技人才重点研发项目国家级大学生创新创业训练计划项目山西医科大学汾阳学院重点学科专项基金

202103021233972022030212123512021RC-1-4202215692022B14

2024

中国抗生素杂志
中国医药集团总公司四川抗菌素工业研究所,中国医学科学院医药生物技术研究所

中国抗生素杂志

CSTPCD北大核心
影响因子:1.08
ISSN:1001-8689
年,卷(期):2024.49(2)
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