Assessment of the diversity and antibacterial potential of culturable actinomycetes in the Kumtag Desert
Desert soil contains abundant actinomycetes,especially many rare strains,thus,it is an ideal habitat for excavating new actinomycete resources.In this study,the screening for antibacterial and culturable actinomycetes isolated from Kumtag Desert soil was carried out to provide strains for developing and utilizing actinomycete resources in this area.Actinomycete diversity was analyzed based on the 16S rRNA gene sequence of isolated strains;From 4 soil samples,370 actinomycete strains,distributed in 22 genera of 12 families and 11 orders were isolated.Amongst them,Streptomyces and Saccharothrix were the dominant genera,accounting for 63％and 13.8％of the isolated actinomycete strains,respectively.Fourteen potential new actinomycete strains(16S rRNA gene sequence similarity less than 98.65％)were fermented in Gauze's synthetic,ISP2 and TSB broth media,respectively.Their fermentation broth was extracted for bacteriostatic activity screening,showing that fermentation products of 11 potential new species had broad-spectrum antibacterial activity.This study indicated that the Kumtag Desert was rich in actinomycete resources,for which potential new species of actinomycetes and antibiotics could be discovered.
NETL
NSTL
万方数据
