Study on antibiotic resistance of culturable Bacteroides from the intestinal tract of children in the Affiliated Hospital of Inner Mongolia Medical University
Objective This study aimed to explore the antibiotic resistance of culturable Bacteroides in children's intestinal tracts in Hohhot.Methods The faecal samples and clinical data of pediatric patients were collected in the Affiliated Hospital of Inner Mongolia Medical University from March 2021 to September 2022.Both the VITEK-2 compact automated microbiology system with anaerobes and coryne bacterium(ANC)cards and MALDI-TOF MS were used to identify and isolate the Bacteroides.The susceptibility of Bacteroides to multiple antibiotics was determined using the agar dilution method.Results A total of 219 Bacteroides isolates were collected in this study,which showed high resistance to a variety of antibiotics.They were highly resistant to penicillin,amoxicillin,clindamycin,ticacillin and piperacillin,with resistance rates of 100％,97.7％,97.3％,70.3％and 68.0％,respectively.Cefotetan,cefoxitin,amoxicillin+clavulanate and imipenem are moderately resistant,with resistance rates of 50.2％,36.1％,17.8％and 12.3％,respectively.Mild resistance to metronidazole,ticacillin+clavulanate,piperacillin+tazobactam and chloramphenicol,the resistance rates were 9.1％,5.5％,4.6％and 0.9％,respectively.Conclusion Culturable Bacteroidetes in children's intestinal tracts in Hohhot had high resistance to a variety of commonly used antibiotics,especially penicillin,amoxicillin,clindamycin,ticacillin and piperacillin.Among them,Bacteroides fragilis had a higher resistance rate to cefotetam,cefoxitin and imipenem than other Bacteroidetes.The resistance rate of children with a history of broad-spectrum antibiotics to piperacillin,cefotetan,ticacillin,cefoxitin and imipenem was higher than that of children without a history of antibiotic usage,and the resistance of Bacteroides isolated from the intestinal tract of elder children to cefoxitin and imipenem was higher than that of younger children.The resistance of Bacteroides in Chinese children might be seriously underestimated.Systematic monitoring of antibiotic susceptibility tests for Bacteroides in children should be strengthened in different areas.
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