Diversity and antibacterial activity of endophytic fungi from bryophyte species in Jinggangshan area
Objective To understand the diversity and community structure characteristics of endophytic fungi in mosses in the Jinggangshan area,and lay a foundation for the study of antibiotics for endophytic fungi from special habitat sources.Methods Culturable methods were used to isolate endophytic fungi.The isolated strains were identified based on morphological characteristics and ITS-rDNA,and the paper diffusion method was applied for antibacterial activity screening.Results A total of 232 cultivable endophytic fungi were purified from four bryophyte species,with isolation frequencies and colonization rates ranging from 1.84～2.88 and 95.65％～100％,respectively.Molecular biology characteristics showed that the endophytic fungi belonged to 38 species,among which penicillium was the dominant endophytic fungi genera of the four bryophyte species.However,the dominant species of each type in bryophytes were different,demonstrating host specificity.The biological activity results showed that the static fermentation products exhibited antibacterial activity,with a comprehensive activity rate of 21.55％.Conclusion This study indicated that the endophytic fungi had generous species diversities,and their secondary metabolites showed great antibacterial activity and potential research value for antibiotic products.
NETL
NSTL
万方数据
