清肺饮治疗急性咽炎模型大鼠的药效及代谢组学研究
Pharmacodynamics and metabonomics of Qingfei decoction in treating acute pharyngitis model rats
雷燕莉 1唐焓嫣 1时政 2刘日慧 1邓梦雨 1吴磊 1刘涛3
作者信息
- 1. 成都大学药学院,成都 610106
- 2. 成都大学附属医院,成都 610081
- 3. 成都大学食品与生物工程学院,成都 610106;四川省抗病毒中药产业化工程技术研究中心,成都 610106
- 折叠
摘要
目的 研究清肺饮对急性咽炎大鼠的药效作用,阐明其作用机制.方法 在清肺饮治疗氨水诱导的急性咽炎大鼠药效试验基础上,通过UPLC-Q-TOF-MS代谢组学技术,结合多元数据统计筛选清肺饮治疗氨水诱导的急性咽炎大鼠的潜在生物标志物,进一步分析相关代谢通路.结果 血常规、血清炎性因子、病理切片结果均表明清肺饮对于氨水诱导的急性咽炎大鼠具有良好的治疗效果.血清代谢组学共筛选出10个清肺饮治疗急性咽炎的内源性生物标志物,可能通过牛磺酸和次牛磺酸代谢,初级胆汁酸生物合成,缬氨酸、亮氨酸和异亮氨酸降解和嘧啶代谢4条重要代谢通路发挥药效.结论 清肺饮具有治疗急性咽炎的作用,可能的机制为调控牛磺酸和次牛磺酸代谢,初级胆汁酸生物合成,缬氨酸、亮氨酸和异亮氨酸降解和嘧啶代谢4条重要代谢通路发挥药效,为临床进一步应用该药提供参考.
Abstract
Objective To study the pharmacodynamic effect of Qingfei decoction on acute pharyngitis rats and clarify its mechanism.Methods Based on the efficacy test of Qingfei decoction in treating acute pharyngitis induced by ammonia water in rats,the potential biomarkers of Qingfei decoction in treating acute pharyngitis induced by ammonia water in rats were screened by UPLC-Q-TOF-MS metabonomics technology combined with multivariate data statistics,and the related metabolic pathways were further analyzed.Results The results of the blood routine,serum inflammatory factors,and pathological section showed that Qingfei decoction had a good therapeutic effect on acute pharyngitis induced by ammonia water in rats.Ten endogenous biomarkers of Qingfei decoction in the treatment of acute pharyngitis were screened out by serum metabonomics,which may play a role through four important metabolic pathways:Taurine and hypotaurine metabolism,primary bile acid biosynthesis,valine,leucine and isoleucine degradation,and pyrimidine metabolism.Conclusion Qingfei decoction has the effect of treating acute pharyngitis,and the possible mechanisms are regulation of taurine and hypotaurine metabolism,biosynthesis of primary bile acids,degradation of valine,leucine and isoleucine,and pyrimidine metabolism,which provide references for further clinical application of this medicine.
关键词
急性咽炎/清肺饮/潜在生物标志物/代谢通路Key words
Acute pharyngitis/Qingfei decoction/Potential biomarkers/Metabolic pathway引用本文复制引用
基金项目
四川省高等教育人才培养质量和教学改革项目(2021-2023)(JG2021-2023)
四川省科技成果转移示范项目(2023ZHCG0071)
出版年
2024
NETL
NSTL
万方数据