赤霉酸GA4+7产生菌的诱变选育研究
Study on mutagenesis and breeding of GA4+7 producing strain
徐旭 1左建英 1熊仁科1
作者信息
- 1. 四川龙蟒福生科技有限责任公司,眉山 620000
- 折叠
摘要
将GA4+7产生菌制备菌丝悬液和原生质体悬液,用紫外照射叠加氯化锂诱变的方法进行迭代诱变,诱变处理后的菌液涂布平板,通过摇瓶培养筛选平板上的单菌落,最后得到一株突变菌GX34-4,相比出发菌株GA4+7,GX34-4的摇瓶效价提高约25％,50 L发酵罐效价提高约40％.与出发菌株的外观和效价不稳定性相比,突变株GX34-4遗传稳定性较好,连续传代5次,产量无明显降低,在50L发酵罐上亦表现稳定.
Abstract
The GA4+7-producing strain was used to prepare mycelium suspension and protoplast suspension,and the iterative mutagenesis was carried out by ultraviolet irradiation and lithium chloride mutagenesis.The mutagenesis-treated bacterial solution was coated on a plate,and the single colonies on the plate were screened by shaking bottle culture.Finally,strain GX34-4 was obtained.Compared to the starting strain GA4+7,the titer of GX34-4 in the shake flask was increased by about 25％,and the titer in the 50 L fermenter was increased by about 40％.The appearance and titer of the starting strain were unstable.The genetic stability of mutant GX34-4 was good,and the yield of mutant GX34-4 was not significantly reduced after five successive generations.which was also stable in a 50 L fermenter.
关键词
原生质体/紫外线/氯化锂/迭代诱变Key words
Protoplast/Ultraviolet irradiation/Lithium chloride/Iterative mutagenesis引用本文复制引用
出版年
2024
NETL
NSTL
万方数据