Objective To improve the production of epothilone B by screening of epothilone producing strains and optimizing the fermentation medium.Methods The secondary metabolites were identified by HPLC and TLC,and the epothilone B producing bacteria were screened out from the culture collection in our laboratory.The fermentation medium components were optimized by a single factor experiment and an orthogonal experiment.Results A Myxobacterium A96-1 producing epothilone B was obtained.The optimized fermentation medium consisted of potato starch(6 g/L),corn slurry dry powder(2 g/L),CaCl2(2 g/L),MgSO4.7H2O(3 g/L),trace element solution(1 mL/L),resin addition(2％).Under the conditions,the fermentation yield of this strain of epothilone B was 251.4 mg/L,which was 3.83 times higher than before optimization.Conclusion The production of epothilone B was significantly increased through strain screening and optimized medium,which provided a reference for the future resource development and utilization and industrialization of epothilone B.
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