首页|星孢菌素产生菌Streptomyces sp.FIM18-327基因组的测序与遗传操作体系的建立

星孢菌素产生菌Streptomyces sp.FIM18-327基因组的测序与遗传操作体系的建立

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目的 通过基因组测序明确星孢菌素高产菌株Streptomyces sp.FIM18-327遗传背景,并建立该菌的遗传操作体系.方法 通过第二代和第三代DNA测序技术相结合的策略对Streptomyces sp.FIM18-327进行全基因组测序并组装,利用antiSMASH软件预测该菌的次级代谢产物生物合成基因簇分布.通过优化接合转移载体、培养基种类及镁离子浓度,建立了Streptomyces sp.FIM 18-327的遗传操作体系.结果 Streptomyces sp.FIM 18-327基因组大小为9.73 Mb,由一条染色体、两个线性质粒及一个环状质粒组成.其中染色体大小为9.48 Mb,GC含量70.12%,包含了8431个蛋白编码基因与34个次级代谢产物生物合成基因簇;以pFIM4123为载体,MS为接合转移培养基,在MS中添加MgCl2至终浓度为20mmol/L时,接合转移的效率达3.2×10-6.结论 Streptomyces sp.FIM 18-327基因组信息的解析及遗传操作体系的建立,为该菌的进一步开发与应用奠定了基础.
Genome sequencing of staurosporine-producing strain Streptomyces sp.FIM18-327 and establishment of its genetic manipulation system
Objective To elucidate the genetic background of the high-producing staurosporine strain Streptomyces sp.FIM 18-327 and establish the genetic manipulation system for this strain.Methods The whole genome of Streptomyces sp.FIM 18-327 was sequenced and assembled using both PacBio Sequel Ⅱ and Illumina NovaSeq platforms,and the secondary metabolite biosynthesis gene clusters in the strain were predicted by antiSMASH.The genetic manipulation system of Streptomyces sp.FIM 18-327 was established by investigating the conjugation transfer carrier,the type of culture medium and magnesium ion concentration.Results The genome size of this strain was 9.73 Mb,which consists of one chromosome,two linear plasmids and one circular plasmid.The chromosome size of Streptomyces sp.FIM18-327 was 9.48 Mb,with a GC content of 70.12%,containing 8,431 protein-coding genes and 34 secondary metabolite biosynthetic gene clusters.With pFIM4123 as the carrier,the efficiency of conjugation transfer reached 3.2× 10-6,when MgCl2 was added to MS medium at the final concentration of 20 mmol/L.Conclusion The analysis of genomic information for Streptomyces sp.FIM18-327 and the establishment of a genetic manipulation system have laid the foundation for the further development and application of this strain.

StaurosporineWhole genome sequencingMetabolic pathwaysConjugation transfer

方志锴、谢立君、林鹏辉、林如、周剑、江红

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福建省微生物研究所,福建省新药(微生物)筛选重点实验室,福州 350007

福建医科大学药学院,福州 350122

星孢菌素 全基因组测序 代谢途径 接合转移

福建省社会发展引导性(重点)项目福州市科技计划项目福建省公益类科研院所专项中央引导地方科技发展专项福建省自然科学基金

2021 Y00442021-P-0262020R100500112021L30162022J01387

2024

中国抗生素杂志
中国医药集团总公司四川抗菌素工业研究所,中国医学科学院医药生物技术研究所

中国抗生素杂志

CSTPCD北大核心
影响因子:1.08
ISSN:1001-8689
年,卷(期):2024.49(9)