Genome sequencing of staurosporine-producing strain Streptomyces sp.FIM18-327 and establishment of its genetic manipulation system
Objective To elucidate the genetic background of the high-producing staurosporine strain Streptomyces sp.FIM 18-327 and establish the genetic manipulation system for this strain.Methods The whole genome of Streptomyces sp.FIM 18-327 was sequenced and assembled using both PacBio Sequel Ⅱ and Illumina NovaSeq platforms,and the secondary metabolite biosynthesis gene clusters in the strain were predicted by antiSMASH.The genetic manipulation system of Streptomyces sp.FIM 18-327 was established by investigating the conjugation transfer carrier,the type of culture medium and magnesium ion concentration.Results The genome size of this strain was 9.73 Mb,which consists of one chromosome,two linear plasmids and one circular plasmid.The chromosome size of Streptomyces sp.FIM18-327 was 9.48 Mb,with a GC content of 70.12%,containing 8,431 protein-coding genes and 34 secondary metabolite biosynthetic gene clusters.With pFIM4123 as the carrier,the efficiency of conjugation transfer reached 3.2× 10-6,when MgCl2 was added to MS medium at the final concentration of 20 mmol/L.Conclusion The analysis of genomic information for Streptomyces sp.FIM18-327 and the establishment of a genetic manipulation system have laid the foundation for the further development and application of this strain.
StaurosporineWhole genome sequencingMetabolic pathwaysConjugation transfer