Objective To isolate and identify FIM-R140,a metabolite from the broth of the sirolimus producing strain Streptomyces hygroscopicus FC904.Methods FIM-R140 was separated and purified by silica gel chromatography and preparative liquid chromatography.Its chemical structure was determined by physicochemical properties and spectral analysis including IR,UV,NMR,and HRMS.The anti-cancer activity of FIM-R140 was determined by the SRB method in vitro,and the antifungal activity was evaluated by the serial two-fold broth dilution method.Results and Conclusion FIM-R140 with HPLC of 97.05％was obtained,and it was identified as 31-epi-rapamycin,a sirolimus isomer,which was reported for the first time.The anti-cancer activity and anti-fungus activity of FIM-R140 were slightly weaker than those of sirolimus.
关键词
西罗莫司/31-差向雷帕霉素/吸水链霉菌FC904/分离纯化/结构鉴定
Key words
Sirolimus/31-epi-rapamycin/Streptomyces hygroscopicus FC904/Isolation and purification/Structure identification
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