Analysis of drug resistance and disinfectant-resistant genes of Pseudomonas aeruginosa isolated from geriatric hospital
Objective This study investigated the drug resistance and distribution of disinfectant-resistant genes among Pseudomonas aeruginosa(PA)isolated from geriatric patients.Methods A total of 111 clinical strains of PA were isolated from geriatric patients from 2019 to 2022.The bacterial identification and antimicrobial susceptibility testing were carried out with VITEK 2 Compact.Then disinfectant-resistant genes were screened by PCR among these strains.Finally,SPSS 21.0 was used to analyze the results.Results Among the 15 drugs,levofloxaxcin had the highest drug resistance rate(30.63％)to PA,while polymyxin B had the lowest resistance rate(1.80％).The proportion of CRPA was 23.42％(26/111),and the proportion of MDR-PA was 20.72％(23/111).Three disinfectant resistance genes were detected:qacE △1-sulI,emrE and merA.The positive rates of the three were 16.22％(18/111),91.89％(102/111),and 46.85％(52/111),respectively.The situation where no disinfectant resistance gene was detected only occurred in three PA strains.The most common mode of carrying disinfectant resistance genes was pattern c(41.44％,46/111);next was pattern f(36.94％,41/111).There was a significant difference in the positive rate of qacE △1-sulI between the CRPA group and the non-CRPA group(P),and between the MDR group and the non-MDR group.In addition,the rate of simultaneous detection of qacE △1-sulI,emrE and merA in the MDR group was significantly higher than that in the non-MDR group.Conclusion The proportion of strains carrying disinfectant-resistant genes in PA isolated from elderly patients was high.There might be a certain correlation between carrying qacE △1-sulI and drug resistance.Clinical practice should require the scientific and rational use of disinfectants and antibiotics to minimize bacterial resistance to both.
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