Drug resistance and resistance gene analysis of Helicobacter pylori in Zibo City
Objective The detection rate of Helicobacter pylori(Hp)in gastric mucosal tissue samples was compared between the bacterial culture method and the nucleic acid detection method.The consistency of the resistance genotypes of Hp to clarithromycin and levofloxacin was compared with that of the"gold standard"culture method to evaluate the value of rapid detection for Hp to clarithromycin and levofloxacin resistance.Methods A total of 84 patients with positive breath tests and one or more quadruple eradications of Hp from January 2022 to February 2023 in the Department of Gastroenterology,Zibo First Hospital,were enrolled in the study.Gastric mucosal tissue samples were taken for Hp culture,Hp nucleic acid detection,clarithromycin and levofloxacin resistance gene detection,and isolated strains were entered testing for resistant phenotypes is performed.Results Of the 84 gastric mucosal tissue samples,60(71.43％)positive and 24(28.57％)negative Hp were detected by the culture method,while 79(94.05％)positive and 5(5.95％)negative Hp were detected by the nucleic acid detection method,and the difference was statistically significant(P＜0.001).The highest resistance rate to metronidazole was 88.3％in 60 positive Hp cultures,followed by levofloxacin and clarithromycin(73.3％),tetracycline and furazolidone(1.7％).No strains resistant to rifampicin and amoxicillin were found,and the susceptibility to antibiotics from high to low was rifampicin,amoxicillin,furazolidone,tetracycline,clarithromycin,levofloxacin and metronidazole.The consistent rate of 23S rDNA mutation of Hp nikramycin gene detected by nucleic acid assay was 91.67％,and that of 261A and 271T mutation of Hp levofloxacin resistance gene detected by nucleic acid assay was 96.67％.Conclusion Hp resistance to clarithromycin and levofloxacin had exceeded 70％.As a result,it was not recommended to use it in the treatment of patients who have failed the first eradication treatment in Zibo.Nucleic acid testing for Hp and resistance genes was consistent with culture,and it can be used for rapid detection of resistance to clarithromycin and levofloxacin.
万方数据
维普
