Epidemiological profiles and drug resistance analysis of vancomycin-resistant Enterococcus faecium clinical isolates
Objective To explore the epidemiology and drug resistance of vancomycin-resistant Enterococcus faecalis in our center and provide a scientific basis for rational drug use and infection control in clinical practice.Methods A retrospective analysis was conducted on 104 non-repetitive vancomycin-resistant Enterococcus faecalis(VRE-fm)strains clinically isolated from the First Affiliated Hospital of Sun Yat-sen University from January 2017 to June 2023.The patient's basic information,sample type distributions,resistance rates to different antibiotics,and resistance and virulence genes carried by the strains were analyzed.Results A total of 104 non-repetitive VRE-fm strains were isolated,with 63.4％(66/104)of patients aged ≥60 years old;VRE-fm strains were mainly isolated from urine(54.8％),drainage(14.4％),and blood(7.7％).The results of the microbroth dilution method drug sensitivity test showed that 104 strains of VRE-fm were all sensitive to linezolid,and the sensitivity rate to tigecycline was 98.1％,with poor sensitivity to other antibacterial drugs.The whole genome sequencing results of 47 VRE-fm strains in our center showed that they all carried vanA resistance genes,but no vanB or vanM resistance genes were detected.All 47 VRE-fm strains carried acm virulence genes,with only 1 VRE-fm strain carrying esp virulence genes,and all other virulence genes were negative.Conclusion Currently,VRE-fm strains exhibit severe drug resistance,typically carrying both drug resistance genes and virulence genes.Consequently,the number of drugs available for clinical treatment of VRE-fm infections is extremely limited.Hence,it is imperative to enhance the management of the use of glycopeptide antimicrobial drugs such as vancomycin and strive to develop and implement infection control methods to effectively prevent the further occurrence and spread of VRE-fm.
万方数据
维普
