Gene cluster analysis and metabolic product isolation of Streptomyces sp.TRM76323 in the rhizosphere soil of Tamarix
Objective This study analyzed the secondary metabolite biosynthesis gene clusters of Streptomyces sp.TRM76323 on the basis of its genomic sequence information,laying the foundation for the discovery of potential new antibiotics.Method The whole genome sequencing of the strain TRM76323.was performed by Illumina Novaseq.Additionally,antiSMASH,CARD,OrthoFinder and Pepperan softwares were used for genome analysis to predict metabolic potential,resistance genes,and genomic differences.These findings provided important theoretical guidance for the discovery of new natural products from microbial sources.Furthermore,modern isolation techniques were utilized to obtain metabolites.Results The genome sequence of the TRM76323 strain consisted of 7,338,911-base pairs,with a G+C content of 72.1％.A total of 31 biosynthetic gene clusters were identified,including resistance genes such as glycopeptide resistance.β-Carbonyl-1H-indole-3-propanal,cyclic dipeptide,and dibutyl phthalate were isolated from this strain.Conclusion Streptomyces sp.TRM76323,found in the rhizosphere soil of Tamarix,possessed a diverse clusters of secondary metabolite biosynthesis genes.This strain had the potential to produce various secondary metabolites and was worth further exploration for the discovery of new antibiotics.
Rhizosphere of Tamarix chinensisStreptomycesWhole genome sequencingSecondary metabolites
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