Secondary metabolites of Streptomyces sp.10-37 from Xizang
Objective This research studied the secondary metabolites of Streptomyces sp.10-37 collected in Xizang.Methods Under the guidance of the molecular network,the compounds were separated and purified by HW40 C gel columns,flash rapid liquid separation and preparation chromatographs,semi-preparative high-performance liquid chromatography and other methods.The structures of the purified compounds were identified by nuclear magnetic resonance and high-resolution liquid chromatography-mass spectrometry.Finally,the antibacterial activity was determined by the microbroth dilution method.Results Four metabolites were isolated and identified from the mycelium extract of Streptomyces sp.10-37,including two new compounds,oxazomycin A3(1)and A4(2),as well as two known compounds,oxazomycin A2(3)and oxazomycin B(4).Compounds 1 and 2 were a pair of geometric isomers of 3,with the configurations of their diene moieties being(4'E,6'E,8'E)(1),(4'Z,6'E,8'E)(2),(4'Z,6'Z,8'E)(3),and(4'E,6'E,8'E)(4),respectively.Conclusion The geographical advantages of Xizang's special environment,combined with the strategy of molecular network analysis,can better excavate new secondary metabolites.
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