Study on the anti-colon cancer activity of the active fraction from clove PEG-PLGA nanoparticles
Objective Active fraction from clove(AFC)has significant anti-colon cancer activity.Previous studies had prepared AFC-PEG-PLGA nanoparticles,and this study explored the anti-colon cancer activity of AFC-PEG-PLGA nanoparticles in vitro and in vivo.Methods Human colon cancer cells(HCT116,SW620,HCT8 and LOVO)were routinely cultivated;colon cancer cell proliferation inhibition rate and IC50 value using the CCK-8 reagent were detected;and colon cancer cells sensitive to AFC-PEG-PLGA nanoparticles were also screened.The nude mouse model of transplanted HCT116 colon cancer cells was established and randomly divided into a negative control group(0.9％NS),a positive control group(fluorouracil,5-Fu,30.0 mg/kg),a PEG-PLGA empty nanoparticle control group,an AFC(100 mg/kg),and an AFC-PEG-PLGA nanoparticles(100 mg/kg)treatment group.After continuous administration for 5 days per week,the drug was stopped for 2 days,and the drug was administered for 4 weeks.Tumor diameter and body weight were measured every 2～3 days,and tumor volume(TV),relative tumor volume(RTV),and relative tumor proliferation rate(T/C％)were calculated.After the experiment,the animals were euthanized,tumor tissue was removed,tumor weight was measured,and the effect of AFC-PEG-PLGA nanoparticles on the growth of transplanted tumors was tested.Results In vitro experimental results showed that the IC50 of AFC-PEG-PLGA nanoparticles on colon cancer cells HCT116,SW620,HCT8,and LOVO were(99.58±5.48),(145.66±6.96),(162.62±6.58),(229.24±9.25)μg/mL,respectively.The strongest activity was observed in HCT116 cells,which were slightly stronger than AFC.In vivo experimental results showed that after treatment with 5-Fu,AFC,and AFC-PEG-PLGA nanoparticles,the TV,RTV,T/C/％,and tumor weight of nude mice were significantly reduced,with T/C/％being 37.54％,57.39％,and 43.04％,respectively.Conclusion The AFC-PEG-PLGA nanoparticles have strong anti-colon cancer activity in vitro and in vivo,with HCT116 having the strongest effect and stronger activity than AFC.
Active fraction from clovePEG-PLGANanoparticlesColon cancerHCT116
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