Study on screening of active components from Lonicera macranthoides to inhibit carbapenem-resistant Pseudomonas aeruginosa quorum sensing system
Objective To screen and identify the major active components of Lonicera macranthoides that inhibit the quorum sensing(QS)system of carbapenem-resistant Pseudomonas aeruginosa and investigate the inhibitory effects of these components.Methods Virtual screening of antibacterial components was conducted using two docking modules in Discovery Studio to simulate molecular interactions and analyze their binding modes.The inhibitory effects of the screened components on the growth activity and biofilm formation of carbapenem-resistant Pseudomonas aeruginosa were determined.A Caenorhabditis elegans infection model was established to evaluate the therapeutic effects of the drugs.Results All three small molecules exhibited varying degrees of growth inhibition and biofilm formation reduction in carbapenem-resistant Pseudomonas aeruginosa.Furthermore,all three small-molecule drugs significantly improved the survival rate of Caenorhabditis elegans after infection.Conclusion The three small molecule drugs derived from Lonicera macranthoides demonstrated significant inhibitory effects on the growth of carbapenem-resistant Pseudomonas aeruginosa,highlighting their potential as novel CRPA inhibitors targeting the QS system.
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