Research on gene regulation of the synthesis of butenyl-spinosyn by enhancing rhamnose synthetic pathways
Objective This study explored the influence and mechanism of overexpression of rhamnose synthesis genes on the growth of Saccharopolyspora pogona and the synthesis of butenyl-spinosyn.Methods The influence of the rhamnose synthesis genes on growth and secondary metabolism was explored by overexpressing the rhamnose synthesis genes or culturing with various concentrations of rhamnose in S.pogona ASAGF58,A qRT-PCR analysis of 23 structure genes and the genes associated with cell growth was performed to identify the key genes that played an important role in yield.Results By overexpressing genes involved in the rhamnose synthesis pathway,three high-yielding strains Rha1,Rha2,and Rha3 were obtained,resulting in a 1.82 fold,2.12 fold,and 2.54 fold increase in butenyl-spinosy production,respectively.Conclusion The titer of butenyl-spinosyn could be improved by optimizing the supply of rhamnose.And gtt and rhamnosyltransferases busG were inferred to be the key genes affecting the production.
Saccharopolyspora pogonaButenyl-spinosynRhamnose synthesis geneConstruction of engineered strainsHigh-yield mechanism
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