The solid-liquid interface properties;especially at the nanoscale;have always been a great concern to the researchers from fundamental science and materials science.The real existence of nanoscale bubbles is contrary to classical thermodynamics and gas diffusion theory.But after the publication of the first paper on the nanobubble image on 2000;it has been shown to be stable at solid-liquid interfaces or bulk phases.A large number of studies for more than 20 years have showed that their characteristics such as ultra-high stability;nanometer size effect and special biological effect are closely related to the interface.These characteristics determine that it will inevitably bring new application prospects;giving rise to many important applications;such as water environment and soil remediation;agricultural planting;aquaculture;industrial cleaning;chemical catalysis;biomedical treatment and diagnosis;and health preservation.Based on the previous research of this research team;hererin we mainly introduce the overview of nanobubble research;focusing on the basic properties of nanobubbles;their special interfacial effects and biological effects;as well as unsolved mysteries and research prospects in the future.