Organoids are functional tissue complexes derived from stem cell by self-organization under in vitro three-dimensional(3D)culture conditions,which can reflect some of the key structural and functional features of the tissue/organ in vivo.However,the formation and culture of most organoids rely heavily on complex extracellular matrices,such as Matrigel,and the tumor-origin and batch variability of these matrices seriously reduce the reproducibility of organoids culture and the safety in subsequent regenerative medicine applications.In addition,due to the lack of adjustability of physical and chemical properties,the above-mentioned matrices are difficult to meet the needs of personalized and customized biomedical research.On the contrary,the hydrogel with single and clear composition has the characteristics of accurate and adjustable biochemical and biophysical properties,which is promising to address the disadvantages of existing organoid matrix,and further realize the construction of more near-physiological organoid models and facilitate their translational application.In this review,we first introduce the existing systems of organoid formation and culture,as well as common hydrogel materials used in organoids research.Then,we introduce the engineering method of hydrogel materials and its representative application in organoids research.Finally,we discuss the potential and future challenges of functional hydrogels in organoid research and translational applications,to provide reference for the utilization of such hydrogels in organoid construction,culture and in-depth research.
hydrogelorganoidbiomaterialstem cellsregenerative medicine
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