Chemical tools capable of probing the dynamic glycosylation/de-glycosylation processes and sugar-protein interactions in-situ
With the rapid advancement of human genomics and proteomics,the study of human glycomics has recently become the focus of many chemists and biologists in the 21st century.Compared to those developed for genomic and proteomic studies,chemical tools for the effective study of the glycomics are much fewer.This account will summarize our recent progress in the development of novel chemical probes for the in-situ detection of the glycosylation/de-glycosylation processes of biomolecules and sugar-protein interactions.We will discuss the principles to design fluorescent probes for the super-resolution imaging of sugar-mediated glycobiological processes in cells,and homogeneous fluorogenic arrays for the high-throughput screening of sugar-protein interactions in a label-free manner.A perspective is also given with respect to the future directions of developing chemical tools for the study of glycobiology.
chemical biologyglycomicsfluorescent probedynamicin-situliving cells
万方数据
维普
