Proteins constitute the most important molecular machines within biological organisms.Understanding the dynamic properties of protein molecular machines and revealing their working mechanisms are crucial for the regulation of their functions.This article provides a comprehensive and systematic review of the research methods for the structural dynamics of protein molecular machines.It delves into the characteristics and applications of different methods from perspectives such as spatial resolution,time-scale,in vitro versus in vivo.Additionally,the review highlights the advantages and limitations of individual methods as well as strategies for an integrated approach.It also looks forward to the future development on the structural dynamics of protein molecular machines and provides authoritative viewpoints.
关键词
蛋白质机器/动态结构/多方法整合/时空尺度
Key words
protein machinery/structural dynamics/integration of multiple methods/spatiotemporal scale
维普
万方数据