Application of Tchebichef image moment method for simultaneous quantitative analysis of multiple components in B-vitamins
The serious overlap of the spectra signals of multiple components with similar chemical structures makes the traditional quantitative analysis methods still have shortcomings for multi-component quantitative analysis of complex systems.The chemometrics methods could separate the characteristic information of the target components and eliminate interference,realizing the quantitative analysis of multi-components at the same time.Herein,the Tchebichef image moment method was employed to establish the quantitative models of Vitamin B1(VB1),Vitamin B2(VB2)and Vitamin B6(VB6)in B-vitamins based on three-dimensional fluorescence spectra.The established models have satisfactory correlation coefficient(R2),adjusted correlation coefficient(Radj2)and root mean square error(RMSE).At the same time,the prediction correlation coefficients(Rp2)of the three models are above 0.9379,indicating that the established quantitative models have high accuracy,reliability and good prediction ability.Finally,the quantitative models have been successfully applied in the determination of three components of B-vitamin complex tablets produced by different manufacturers.It could be seen that the Tchebichef image moment method can solve the signal overlap and interference of multi-target components and provides an important basis for the simultaneous quantitative analysis of multi-components in complex systems.