Ginsenoside Rg1 reduces the inflammatory response of microglia after oxygen glucose deprivation/resupply by inhibiting the NLRP3 inflammasome pathway
Objective To investigate the effect of ginsenoside Rg1 on the inflammatory response of microglia cells after oxygen-glucose deprivation/resupply injury through NLRP3 inflammasome pathway,and to further investigate its anti-inflammatory mechanism.Methods BV-2 microglia were randomly divided into six groups:No treatment group(Con),oxygen glucose deprivation/resupply group(OGD/R),ginsenoside Rg1 low,medium and high dose groups(0.1,0.2,0.4 mmol/L,Rg1L,Rg1M,Rg1H),MCC950 control group(0.05 mmol/L,MCC950).The cell proliferation was detected by CCK8.Immunofluorescence and western blotting were used to detect the expression of NLRP3 inflammasome-related protein in BV-2 microglia after treatment with different concentrations of ginsenoside Rg1 and MCC950 for 48 h.The expression levels of IL-1β and IL-18 in BV-2 microglia were detected by ELISA.Results Compared with Con group,Iba-1 green fluorescence was observed in the cytoplasm of BV-2 microglia cells treated with hypoxia and glucose deficiency/resupply.After OGD/R treatment of BV-2 microglia for 2h,the proliferation rate of BV-2 microglia cells showed an obvious downward trend after 48 h culture with different concentrations of ginsenoside Rg1 and MCC950.There were significant positive expressions of NLRP3,ASC,Caspase-1,IL-1β and IL-18 in the OGD/R group.The NLRP3 protein expression in Rg1L,Rg1M and Rg1H groups was significant1y decreased,and the expression decreased with the increasing of drug concentration.The results showed that compared with OGD/R group,ginsenoside Rg1 and MCC950 inhibited the expression of NLRP3 in activated BV-2 microglia cells(P＜0.01).Conclusions Ginsenoside Rg1 may inhibit the expression levels of IL-1β and IL-18,thus inhibiting the inflammatory response by inhibiting the expressions of NLRP3,ASC,Caspase-1 and interfering with NLRP3 inflammasome synthesis..
Ginsenoside Rg1Oxygen and glucose deprivation/resupplyMicrogliaInflammationNLRP3
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